White leg shrimp (Litopenaeus vannamei), also known as Pacific white shrimp, is a variety of prawn of the eastern Pacific Ocean commonly caught or farmed for food. Litopenaeus vannamei grows to a maximum length of 230 millimeters (9.1 in),. It is restricted to areas where the water temperature remains above 20 °C (68 °F) throughout the year.
Slow Sand Filter - used in water purification for treating raw water to produce a potable product. They are typically 1 to 2 meters deep, can be rectangular or cylindrical in cross section and are used primarily to treat surface water. Water storage and filtration tank - The water storage tank is normally elevated to effectively distribute water by gravity to the hatchery. The water storage tank capacity should be at least 20% of the larval rearing tanks. Storage tanks are normally constructed out of reinforced concrete to withstand the water pressure. Effluent Treatment System (ETP) - All the waste water is collected in the Effluent Treatment Plant and is applied 50ppm Chlorine. It is then subjected to aeration for about 1 hour and released into the sea.
BROOD STOCK MANAGEMENT
Method involves manipulating environmental factors surrounding the brood stock to ensure maximum survival and increase fecundity. A matured, full grown Broods measuring 36 to 40 gms were imported from United States for reproduction, under goes a detailed examination by Aquatic Quarantine Facility, Chennai, to facilitate a regulated mode of introduction of non-native L. vannamei into India.
Brood stock management
Eye stalk ablation
Matured male and female brood stocks are reared about 20-25 days in holding tanks to recover from transport stress & to adopt climate change. They are feed with warms, Squids, Oysters supplemented with feed additives. Mating occurs during the time when the broods are newly molted. Succeeding maturation females release developing embryos into the water. The first larval stage is known as Nauplius
STALK ABLATION Removal of one (unilateral) or both (bilateral) eye stalks on female shrimps to stimulate the development of mature ovaries. Eye stalk ablated shrimps respond to their operation with a rapid and unstoppable gonadal development thus augmenting total egg production in given time.
Reverse transcription polymerase chain reaction (RT-PCR), a variant of polymerase chaing reaction (PCR), is a technique commonly used in molecular biology to detect RNA expression
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